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    Amino Acids and Peptides

    簡要描述:The column is suitable for the separation of derivatives of amino acids (A) and peptides (P). The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSY

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    • 更新時間:2024-08-07
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    詳細介紹

    Amino Acid and Peptide Analysis

     

    UltraSep ESD AP

      

    The column is suitable for the separation of derivatives of amino acids (A) and peptides (P). The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSYLchloride shows advantages.

    Comparison of different procedures for derivatization

    Procedure

    Detection

    Limit of detection

    Remarks

    DABS

    Absorption
    436 nm

    < 1 pmol

    Stable derivatives; primary and secondary amino acids can be determined

    OPA

    Fluorescence
    330/440 nm

    < 1 pmol

    Pro, Hypro and Cys cannot be determined 

    FMOC

    Fluorescence
    265/315 nm

    < 1 pmol

    His and Cys will be detected only in part

    DABS = DABSYLchloride (Dimethylaminoazobenzenesulfonylchloride)
    OPA = o-Phthalaldehyde
    FMOC = 9-Fluorenylmethoxycarbonylchloride

     

    Examples of Analyzed Compounds

    Analyzed in Application

    Go to

     

    Alanine (Ala)

    AA

     

     

    Arginine (Arg)

    AA

    AA

     

    Aspartic acid (Asp)

    AA

     

     

    Cystine (Cys2)

    AA

    EPHARM6

     

    Glutamic acid (Glu)

    AA

     

     

    Glycine (Gly)

    AA

     

     

    Glutathione

    EPHARM6

     
     

    Histidine (His)

    AA

     

     

    Isoleucine (Ile)

    AA

     

     

    Leucine (Leu)

    AA

     

     

    Lysine (Lys)

    AA

     

     

    Methionine (Met)

    AA

     

     

    Ornithine (Orn)

    AA

     

     

    Phenylalanine (Phe)

    AA

     

     

    Proline (Pro)

    AA

     

     

    Serine (Ser)

    AA

     

     

    Threonine (Thr)

    AA

     

     

    Tyrosine (Tyr)

    AA

     

     

    Valine (Val)

    AA

     

     

    COLUMNS and  PRICES  ()
     Column size (mm)
    250 x 4250 x 3250 x 2125 x 4125 x 3125 x 2
    UltraSep ESD AP360.00340.00355.00320.00295.00290.00
     

    Size (mm)

     
    10 x 410 x 310 x 2
    Precolumn cartridges
    (Price per pack = 5 pieces)
    170.00170.00170.00

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